Introduction
The new deltaEmerald allows simultaneous SRS imaging of two vibrational bands with its revolutionary dual color SRS (DC-SRS) scheme. It is aimed at users at the forefront of SRS microscopy, giving the best signal-to-noise performance and the capability of background subtraction as well as simultaneous imaging of two vibrational bands.
Two Stokes pulses, separated by 85 cm-1 and modulated at different frequencies are overlapped with the tunable Pump pulse.
The pulse length of about 1 ps, 10 cm-1 … 15 cm-1 bandwidth and several 100 mW output power in each beam are ideal parameters for Coherent Raman imaging.
The pulses of all three beams are shot noise limited due to solid state laser design and proven OPO technology. The shot noise limit of -162 dBc/Hz above 1 MHz (limited by the noise measurement setup with 4.8 mA) allows for fast image acquisition and superior signal-to-noise ratio.
Fully automated tuning, power control and temporal and spatial overlap of all three beams are given.
Additionally a ~100 fs output at 1030 nm is provided for efficient SHG and two-photon imaging.
For more details regarding Coherent Raman Microscopy, please look here.
At a Glance
- Innovative SRS scheme for simultaneous excitation of two vibrational bands
- Tunable Pump and both Stokes pulses are spatially and temporally overlapped
- Spectral distance of Stokes beams 85 cm-1
- Proven OPO technology with shot noise limited performance with -162 dBc/Hz above 1 MHz
- Additional femtosecond output at 1030 nm beam for optimum SHG and TPF excitation
Applications
- Simultaneous imaging of CH2 & CH3 for nondestructive and fast Coherent Raman histology. The 85 cm-1 energy difference is chosen to address CH2 & CH3 with 2845 cm-1 and 2930 cm-1 at their peak position.
- Background removal of non-resonant artefacts, especially important for weak signals in the fingerprint region
- Cream spreading quantification, Drug penetration, Metabolic imaging, Microplastics discrimination, Plant imaging, Cell imaging, Fingerprint imaging, etc.
Specifications
Applications
deltaEmerald | |
Wavelength 1 (Stokes 1) | 1034 nm |
Wavelength 2 (Stokes 2) | 1025 nm |
Wavelength 3 (Pump - Signal) | 740 nm … 990 nm |
∆v Pump - Stokes | 350 cm-1 … 3800 cm-1 |
Power Stokes 1 | >300 mW (unmodulated), >150 mW modulated (20 MHz) |
Power Stokes 2 | >300 mW (unmodulated), >150 mW modulated (13 MHz) |
Power Pump (Signal) | >400 mW at 800 nm |
Spectral bandwidth | 10 cm-1 … 15 cm-1 (Pump and Stokes) |
Pulse width | 1 ps |
Repetition rate | 80 MHz |
Noise | Shot noise limited (-162 dBc/Hz) >1 MHz for Pump and Stokes |
Beam parameters | |
Pointing stability | <100 µrad per 100 nm |
M² | <1.2 for Pump and Stokes |
Ellipticity | <20% |
Polarization | Linear, horizontal >100 : 1 |
Beam divergence | 0.9 mrad ± 0.2 mrad at 800 nm and Stokes |
Beam waist diameter | 1.3 mm ± 0.2 mm at 800 nm, 1.7 mm ± 0.2 mm for Stokes |
Additional femtosecond output | |
Wavelength | 1030 nm ± 3 nm |
Power | 300 mW |
Pulse width | <200 fs |
Beam divergence | <1.5 mrad |
Features | |
Integrated modulator for Stokes | Built-in EOMs with resonant fixed frequency of 20 MHz and 13 MHz |
Power attenuators | Integrated for Pump and Stokes, independent for each beam |
Delay management | Automated compensation for microscope dispersion |
Connectivity | Ethernet and Software interface TCP/IP |
- Simultaneous imaging of CH2 & CH3 for nondestructive and fast Coherent Raman histology. The 85 cm-1 energy difference is chosen to address CH2 & CH3 with 2845 cm-1 and 2930 cm-1 at their peak position.
- Background removal of non-resonant artefacts, especially important for weak signals in the fingerprint region
- Cream spreading quantification, Drug penetration, Metabolic imaging, Microplastics discrimination, Plant imaging, Cell imaging, Fingerprint imaging, etc.
Datasheets & Brochures
APE deltaEmerald rev.1.0.0 (pdf / english)
or
Email & Phone Contacts
APE has distributors around the world to give you the best support. Choose a country to find your local sales contact:
Dual Color Stimulated Raman Scattering with deltaEmerald full compatible with DC-CRS microscope by Lightcore Technologies.